Development of real-time imaging, spectral flow cytometry and strategically designed fluorochromes
Автор: BD Biosciences
Загружено: 2023-08-24
Просмотров: 3329
Imaging flow cytometry has provided an additional layer of information for the characterization of cells based on the spatial distribution and localization of fluorescence signals. However, camera-based systems have so far been limited by the slow speed of image measurement, which prevented real-time cell sorting based on imaging parameters. In this presentation, we will describe how the BD CellView™ Image Technology was developed to overcome these limitations and enable, for the first time, high-speed image fluorescence cell sorting. We will furthermore describe how this technology was integrated in a 5-laser, 78 detector spectral flow cytometer. Lastly, we will introduce a new family of fluorochromes strategically designed to enable high-parameter flow and image cytometry and minimize the current challenges posed by the limited availability of unique, laser-specific fluorochromes.
For Research Use Only. Not for use in diagnostic or therapeutic procedures
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