How to Create & Use a Local BLAST Database in your Computer/Laptop | Bioinforamtics Tutorials |
Автор: PhDBioinformatics
Загружено: 2025-03-18
Просмотров: 288
🔬 NCBI BLAST+ Installation & Local Database Setup Guide 🖥️
Welcome to this step-by-step tutorial on how to install NCBI BLAST+ on your local PC, create a BLAST database, and execute a query! 🚀 Whether you're a bioinformatician, researcher, or student, this guide will help you run BLAST efficiently on your machine.
📌 What You’ll Learn in This Video:
✅ How to download & install NCBI BLAST+ on your local system
✅ How to create a BLAST database 🗄️
✅ How to run a BLAST query and interpret results
✅ Useful commands & troubleshooting tips
💡 Commands Used in This Video:
Install NCBI BLAST+
sudo apt update
sudo apt install ncbi-blast+
Create a BLAST Database
makeblastdb -in your_sequences.fasta -dbtype nucl -out my_blast_db
Run a BLAST Query, blastn for nucleotide seqs, blalstp for protein sequences
blastn -query query.fasta -db my_blast_db -out results.txt
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