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Postganglionic Sympathetic Neurons Differentiation from hiPSCs | Protocol Preview

Developmental Biology

Education

Lab

Laboratory

Lecture

Method

Protocol

Research

Science

Tutorial

autonomic nervous system

chemically defined stem cell culture

feeder free

human pluripotent stem cells

neural crest

sympathetic nervous system

sympathetic neurons

Автор: JoVE (Journal of Visualized Experiments)

Загружено: 6 сент. 2022 г.

Просмотров: 43 просмотра

Описание:

Watch the Full Video at https://www.jove.com/v/60843/efficien....

Efficient Differentiation of Postganglionic Sympathetic Neurons using Human Pluripotent Stem Cells under Feeder-free and Chemically Defined Culture Conditions - a 2 minute Preview of the Experimental Protocol

Hsueh Fu Wu, Nadja Zeltner
University of Georgia, Center for Molecular Medicine; University of Georgia, Department of Biochemistry and Molecular Biology, Franklin College of Arts and Sciences; University of Georgia, Department of Cellular Biology, Franklin College of Arts and Sciences;

In this protocol, we describe a stable, highly efficient differentiation strategy for the generation of postganglionic sympathetic neurons from human pluripotent stem cells. This model will make neurons available for the use of studies of multiple autonomic disorders.

Visit https://www.jove.com?utm_source=youtu... to explore our entire library of 14,000+ videos of laboratory methods and science concepts.

JoVE is the world-leading producer and provider of science videos with the mission to improve scientific research and education. Millions of scientists, educators, and students at 1500+ institutions worldwide, including schools like Harvard, MIT and Stanford benefit from using JoVE's extensive library of 14,000+ videos in their research,education and teaching.

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Postganglionic Sympathetic Neurons Differentiation from hiPSCs | Protocol Preview

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