Column Chromatography

We just learned about thin layer chromatography, which lets us separate the components of a mixture by polarity. But that just separated a tiny bit. We will need to separate entire product mixtures, so that we can isolate a target molecule. That will require column chromatography. Conceptually it's just the same as TLC, but with a column, you load everything you've got right in there, and the mixture separates as it goes down the column. Collect the relevant fractions, evaporate the solvent, and boom! Isolated target molecule. It's a little involved so let's learn how to do it!

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