Jakub Bogusławski "Femtosecond lasers in multiphoton microscopy: from principles to imaging (...)"

A lecture by Jakub Bogusławski (Wrocław University of Science and Technology, Wrocław, Poland)
Femtosecond lasers in multiphoton microscopy: from principles to imaging applications

This lecture is a part of the Candela Open Lectures: Exploring the Ultrafast Optics series.
To learn more about the lecture series, visit the website: https://www.candela.org.pl/ouo_lectures

Abstract

Multiphoton microscopy enables high-resolution, three-dimensional imaging of biological tissues with minimal photodamage — a capability made possible by femtosecond laser sources. In this lecture, the fundamental principles of multiphoton excitation and fluorescence will be introduced, followed by a discussion of the specific laser parameters necessary for effective imaging — including pulse duration, repetition rate, wavelength, and beam quality. Traditional solid-state laser systems, particularly Ti:sapphire lasers, will be presented as benchmark sources, alongside an overview of emerging fiber-based femtosecond lasers that offer enhanced stability, compactness, and cost efficiency. Selected applications in ophthalmology and dermatology will illustrate how advances in ultrafast laser technology continue to enhance the performance and accessibility of multiphoton microscopy.

Lecturer’s bio

Jakub Bogusławski received his Ph.D. in 2018 from Wrocław University of Science and Technology, where he is currently an Assistant Professor at the Faculty of Electronics, Photonics and Microsystems and Principal Investigator of the Ultrafast Biophotonics Laboratory. His research focuses on ultrafast fiber lasers and their applications in biophotonics, particularly in advanced optical imaging.
Following his doctoral studies, he held a postdoctoral position at the International Centre for Translational Eye Research in Warsaw, Poland, which involved frequent research visits to the School of Medicine at the University of California, Irvine (USA). During this time, he collaborated with high-tech companies in the field of medical diagnostics, contributing to the translation of photonic technologies into biomedical applications.
His current work aims to advance ultrafast photonic technologies for biology and medicine, transforming cutting-edge optical methods into practical, accessible tools that accelerate discovery and improve human health.

Video Chapters

0:00 Introduction
1:31 Lecture pt.1
45:44 Q&A Session 1
1:01:20 Lecture pt. 2
1:33:30 Q&A Session 2