Lina Streich: "High-resolution deep brain imaging using adaptive optics three-photon microscopy"

Lina Streich is a Predoctoral Fellow, Prevedel Group at EMBL Heidelberg. The Prevedel group develops new optical techniques for investigating dynamic cellular processes deep inside tissue in vivo.

Abstract: The main hurdles for imaging deep into the intact mammalian brain are tissue scattering and the degradation of image quality due to optical aberrations and motion artefacts. In my talk I will present our work on a minimally invasive intravital imaging methodology that adresses these shortcomings by combining three-photon excitation, indirect adaptive optics correction, and active electrocardiogram gating. I will show how our method can be used to achieve near-diffraction limited resolution up to a depth of 1.2mm in the mouse brain to visualize fine structures such as dendrites and spines in deep cortical and subcortical brain regions. Furthermore, I will also demonstrate the capability of our microscope to record calcium transients in deep-layer astrocytes in-vivo.

Janelia’s Optical Interest Group is currently partnering with EMBL’s Microscopy Optics and Image Analysis interest group (MOIA) in a monthly seminar series. The series is open to members of both institutions and the presentations will be available to the public afterwards.

More information about Janelia’s Optical Interest Group Seminar Series can be found here: https://www.janelia.org/content/optic....