Protein Crystallisation - Fabrice Gorrec

The LMB Crystallisation Facility aids LMB scientists to obtain high quality crystals of purified protein, DNA, RNA, small molecules or any combination thereof for structural biology. Users are able to screen a broad range of initial crystallisation conditions using automated protocol using robots to dispense in pre-made MRC plates. Following initial screening there are several optimisation protocols to aid success.

In this video Fabrice gives background and theory to crystallisation and then details the processes involved in project success including: obtaining more optimal protein samples, setting up the crystallisation experiment; initial screening; assessment of experiments and optimisation protocols and tips.

The LMB Crystallisation Facility is run by Fabrice Gorrec.

Read more about the facility here: https://www2.mrc-lmb.cam.ac.uk/resear...

You can find more videos from the LMB 2021 Biophysics Techniques Lecture series here:
   • Introduction to Biophysical Techniques 2021  

Other playlists you might be interested in:
Next Generation Biophysics Symposium 2020 -    • Next Generation Biophysics Symposium 2020  
Electron Cryo-Microscopy Course 2017 -    • Electron Cryo-microscopy Course 2017  
RNA Seq Course -    • RNA-seq Course 2020  
LMB Crystallography Course 2013 -    • LMB Crystallography Course 2013  

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About the MRC Laboratory of Molecular Biology (LMB):
The LMB is one of the world's leading research institutes. Discoveries and inventions developed at the LMB, for example DNA sequencing and methods to determine the structure of proteins, have revolutionised all areas of biology. Its scientists work to advance understanding of biological processes at the molecular level. This information will help us to understand the workings of complex systems, such as the immune system and the brain, and solve key problems in human health.

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