QIIME2 Part 1: Step-by-Step FASTQ Import & DADA2

QIIME2 Part 1: Step-by-Step FASTQ Import & DADA2
QIIME2 Part 1: Step-by-Step FASTQ Import & DADA2

Welcome to Part 1 of this step-by-step QIIME2 tutorial for 16S rRNA amplicon sequencing analysis.
In this video, you will learn how to install QIIME2, import FASTQ files, perform demultiplexing, run DADA2 denoising, and generate & explore table.qzv, rep-seqs.qzv, and denoising-stats.qzv files using view.qiime2.org.

This beginner-friendly guide is perfect for students, researchers, or anyone starting with microbial community analysis using 16S rRNA sequencing.

⭐ Topics Covered in Part 1:

✔️ Installing and setting up the QIIME2 environment
✔️ Preparing Manifest files
✔️ Importing FASTQ into QIIME2
✔️ Visualizing demultiplexed data (demux.qzv)
✔️ Running DADA2 denoising (paired-end)
✔️ Generating:
 • table.qza / table.qzv
 • rep-seqs.qza / rep-seqs.qzv
 • denoising-stats.qza / denoising-stats.qzv
✔️ How to interpret QIIME2 visualizations (quality plots, ASVs, summary tables)
The commands for a typical fastq file download and qiime2 analysis can be sourced from our github repository:

https://github.com/dr-deepakkukkar/qi...

⭐ Who is this video for?

🔬 Microbiome researchers
🧬 Bioinformatics beginners
📊 Students learning 16S rRNA NGS analysis
💡 Anyone transitioning from wet lab to computational biology

⭐ Part 2 Coming Soon:

Taxonomy assignment, phylogeny, diversity analysis (alpha/beta), and visualizations.

Keywords:
qiime2 tutorial
qiime2 installation
16S rRNA analysis
dada2 qiime2
how to import fastq qiime2
qiime2 demux
microbiome analysis tutorial
feature table qiime2
rep-seqs qiime2
qiime2 for beginners

For any teaching/learning related queries, please contact: [email protected]

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4] Master FASTQ Downloads with SRA Toolkit in Conda | Bioinformatics Made Easy (Hindi)
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