Bioinformatics - Understanding FastQC/MultiQC (Timestamps)

In this video, I thought it would be a good idea to take a step back and start to understand some of the tools that we have used so far - in particular FastQC and it's quality control reports. FastQC provides a nice report for several important metrics about .fastq read files. Additionally, MultiQC makes it easier to see these metrics for all of the files within a project to see if there are any that are behaving much differently than others.

This video uses the FastQC report created in the previous video on the raw read file SRR2121770_1.fastq.gz. This is from this publication:
https://pubmed.ncbi.nlm.nih.gov/26372...

Make sure to check out    • Bioinformatics - SRA Download, QC, and Tri...   for how the files were downloaded and the tools installed/ran.

FastQC manual:
https://dnacore.missouri.edu/PDF/Fast...

Timestamps:
0:01:24 - Basic Statistics
0:04:28 - Per Base Sequence Quality
0:08:04 - Per Tile Sequence Quality
0:11:07 - Per Sequence Quality Scores
0:12:33 - Per Base Sequence Content
0:14:43 - Per Sequence GC Content
0:17:36 - Per Base N Content
0:19:04 - Sequence Length Distribution
0:20:37 - Sequence Duplication Levels
0:23:24 - Over-represented Sequences
0:25:23 - Adapter Content



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Project Github:
https://github.com/ACSoupir/Bioinform...

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