How to plot Volcano Plots with R ggplot | RNA Seq | Gene Expression

This tutorial shows you how to visualize gene expression data by generating volcano plots using R

Download the Rscript for this tutorial:   / 75743948  
Use Galaxy to generate Volcano Plots:    • RNASeq Analysis on Galaxy Tutorials | How ...  

RNA Seq Analysis with DESEq2:   • DESeq2 Tutorial | How I analyze RNA Seq Ge...  
RNA Seq Analysis Tutorials:   • RNA Seq Analysis Tutorials  


*Thank me with a Coffee*: https://www.buymeacoffee.com/informat...
*Tip me on Paypal*: https://www.paypal.com/paypalme/thein...

*One-on-One coaching*: https://calendly.com/bioinformaticscoach
*Consultation: https://calendly.com/bioinformaticscoach

Reach out
[email protected]

Get more bioinformatics tutorials on Patreon:   / bigdataanalytics  


Volcano Plots in R | Bioinformatics | Reproducing Galaxy Tutorials | Beginners| Getting Started


Course Materials
________________________________________________________________________________________________
Download the data here:
https://training.galaxyproject.org/tr...

R Source Code
https://biocorecrg.github.io/CRG_RInt...

How to Install R
________________________________________________________________________________________________
How to install R and R packages using Anaconda
   • Beginner's guide to Install R & R packages...  
How to Install R in Ubuntu
   • Ubuntu Tutorials | How to Install  R  & Rs...  


Useful tutorials
________________________________________________________________________________________________
Gene Expression Analysis
   • How I analyze RNA Seq data using HISAT2 , ...  



Chapters
________________________________________________________________________________________________
00:00 Introduction
01:23 PC Requirement
01:34 Download the example data
04:19 Install packages
06:45 Open R studio
06:55 Set the working directory
07:54 Load the R packages
08:30 Load the data
12:34 Add a new column to label the gene expression status of the genes
21:55 Plot the volcano plot
26:21 Add horizontal and vertical lines to the plots
30:02 Highlight the top genes on the plot
31:22 Sort and select the top genes based on pvalues
35:08 Label the top genes in the dataframe
39:27 Generate the volcano plot and highlight the top genes



R ggplot
R volcano plot


Opening Music
Buddha by Kontekst   / kontekstmusic  
Creative Commons — Attribution-ShareAlike 3.0 Unported — CC BY-SA 3.0
Free Download / Stream: http://bit.ly/2Pe7mBN
Music promoted by Audio Library    • Buddha – Kontekst (No Copyright Music)  










#bioinformaticsforbeginners #bioinformatics #bioinformática #linuxforbeginners #linux