RNA-seq tutorial with DESeq2: Differential gene expression project
Автор: OMGenomics
Загружено: 2024-12-17
Просмотров: 19542
Make your own bioinformatics project that reproduces a differential gene expression analysis using DESeq2 and the Gene Expression Atlas.
Play with this data in Circa: https://circa.omgenomics.com/app/plot...
Timestamps:
0:00 Intro
1:11 Where to find published RNA-seq data
2:25 Download data from the Gene Expression Atlas
4:24 Wrangle the data for DESeq2
7:49 Spot check the data
9:17 Run DESeq2
12:50 More complex design formulas
16:13 What does the ~ mean?
17:32 Compare your results to the Gene Expression Atlas
19:55 Make an MA plot and Volcano plot
21:09 Make a circos plot
R script: https://github.com/omgenomics/youtube...
How the DESeq design formula works: • DESeq2 Design Explained: Understanding Int...
Circa circos plotting software: https://circa.omgenomics.com/?yt=deseq
Try Circa for free on RNA-seq data: https://circa.omgenomics.com/app/plot...
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