P. aeruginosa Culturing and Quantitation of Alginate | Protocol Preview

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Culture of Small Colony Variant of Pseudomonas aeruginosa and Quantitation of its Alginate - a 2 minute Preview of the Experimental Protocol

Roy Al Ahmar, Brandon D. Kirby, Hongwei D. Yu
Marshall University, Department of Biomedical Sciences, Joan C. Edwards School of Medicine; Robert C. Byrd Biotechnology Science Center, Progenesis Technologies LLC; Marshall University, Department of Pediatrics, Joan C. Edwards School of Medicine;

Here, we describe a growth condition to culture the small colony variant of Pseudomonas aeruginosa. We also describe two separate methods for the detection and quantitation of the exopolysaccharide alginate produced by P. aeruginosa using a traditional uronic acid carbazole assay and an alginate-specific monoclonal antibody (mAb) based ELISA.

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