Antibody Affinity Purification: Differences Between Proteins A, G, and L

In this video, we’re diving into affinity purification for antibodies—a powerful and widely used technique in protein biochemistry. We’ll break down how affinity chromatography works, review antibody structure (including heavy and light chains), and compare three essential affinity proteins: Protein A, Protein G, and Protein L. #biochemistry #proteinpurification #labtechniques

🔬 Find helpful resources from GoldBio.com below!

GoldBio Handbooks - https://goldbio.com/handbooks
What is Affinity Purification for Antibodies? - https://www.goldbio.com/blogs/article...
Relative Affinity of Immobilized Protein A and Protein G for Various Species and Subclasses of
Polyclonal and Monoclonal IgGs - https://www.goldbio.com/blogs/documen...
Protein Expression & Purification - https://www.goldbio.com/collections/p...

Related Products
Protein A Magnetic Agarose Beads - https://goldbio.com/products/protein-...
Protein G Magnetic Agarose Beads - https://goldbio.com/products/protein-...
Protein L Magnetic Agarose Beads - https://goldbio.com/products/protein-...

Chapters
Intro 0:00
Affinity purification overview 0:35
What is the structure of an antibody 1:32
What are the 5 classes of antibodies 1:59
Difference between Protein A, Protein G and Protein L 2:36
How to chose the right affinity protein? 3:38
For free handbooks visit goldbio.com 4:39


Like the shirt? Enter to win one of your own! - https://goldbio.com/pages/win-a-goldb...
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Presented by Karen Martin, Edited by Karen Pierce
Gold Biotechnology, Inc. - https://www.goldbio.com
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