Choosing the Right Resin: Anion vs Cation Exchange Explained

Are you new to ion exchange chromatography? This beginner-friendly video explains the essentials of purifying proteins based on their charge. We'll help you understand the difference between anion exchange and cation exchange resins, when to use them, and why you need to consider the isoelectric point (pI) of your protein in order to optimize your buffer conditions.

Chapters
0:00 - Intro
0:21 - What is Ion Exchange Chromatography?
0:33 - Difference between Anion and Cation?
1:34 - Buffer pH in Ion Exchange Chromatography
2:10 - Cation Exchange Resins
3:03 - Anion Exchange Resins
3:33 - Resolution vs Speed

Related Products
5X Phosphate Buffer, pH 8 - https://goldbio.com/product/2075/5x-p...
Plastic Columns - https://goldbio.com/product/14402/pla...
Protein Expression & Purification - https://www.goldbio.com/collection/pr...

🔬 Find helpful resources from GoldBio.com below!
The 2 Common Types of Ion Exchange Chromatography: Anion vs. Cation - https://goldbio.com/articles/article/...
An Overview on Ion-Exchange Chromatography - https://goldbio.com/articles/article/...
Charged up: The Complexities and Significance of Protein Charge in Protein Purification - https://goldbio.com/articles/article/...
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Presented by Karen Martin, Edited by Karen Pierce
Gold Biotechnology, Inc. - https://www.goldbio.com
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