5 Detergents for Cell Lysis/Protein Extraction: CHAPS, Chlorhexidine, Deoxycholic Acid & Digitonin

In this video, we will examine the top five detergents for cell lysis and protein extraction, which are essential components in the process of protein purification. The choice of detergent plays a significant role in disrupting cellular membranes and solubilizing proteins. We will investigate these important detergents: CHAPS, Chlorhexidine, Deoxycholic Acid, and Digitonin. We will discuss their classifications—zwitterionic, nonionic, cationic, and anionic—and how their distinct properties can enhance experimental outcomes. Whether you are working with sensitive cells or challenging proteins, this video aims to equip you with the necessary knowledge to choose the most suitable detergent for your specific research needs. #labtech #biotech

Resources
12 Useful Detergents for Your Experiments - https://goldbio.com/articles/article/...
How Detergents are Extremely Useful in Bioscience Experiments -
https://goldbio.com/articles/article/...
Detergent Selection Guide - https://goldbio.com/documents/4044/De...

Chapters
Intro 0:00
Classification of detergents 0:25
CHAPS 1:22
Chlorhexidine 1:41
Deoxycholic Acid 2:02
Digitonin 2:26
Octylglucoside and Octylthioglucoside 2:45
For more information 3:13

Related Products
GoldBio Detergents & Membrane Agents - https://goldbio.com/collections/deter...
CHAPS - https://goldbio.com/product/3742/chaps
Digitonin - https://goldbio.com/product/1130/digi...


At Goldbio.com
An Overview on Ion-Exchange Chromatography - https://goldbio.com/articles/article/...
The 2 Common Types of Ion Exchange Chromatography: Anion vs. Cation - https://goldbio.com/articles/article/...
Charged up: The Complexities and Significance of Protein Charge in Protein Purification - https://goldbio.com/articles/article/...
_________________________________________________________________________________________

Gold Biotechnology, Inc. - https://www.goldbio.com
At GoldBio, we strive to exceed the expectations of our customers, vendors and strategic partners through quality of products, excellent service and attention to detail. Our sustainable business model and relationships with others in the supply chain allow us to provide researchers and scientists with uniquely low prices, while maintaining the highest quality in the industry. We stand behind all our products to the fullest extent with the hope that they will be used in discoveries that benefit all. Come say hi on other forms of social media!

Linkedin:   / gold-biotechnology-inc-  
Facebook:   / goldbiotechnology  
BlueSky: https://bsky.app/profile/goldbio.bsky...
Instagram:   / goldbiostl