How to do a simple Grain to Grain transfer| Fastest colonization method!

Check out our video on how to make your own Master's Mix 5lb block:    • How to Make Master’s Mix for Growing Mushr...  

Materials Needed:
✅ Fully colonized bag or jar of grain spawn
✅ Sterilized bags/jars of grain (receiving containers)
✅ Alcohol spray (70% isopropyl)
✅ Sterile gloves 🧤 / face mask 😷
✅ Laminar flow hood or still air box (SAB) 🌀
✅ Measuring utensils for transferring grains (or eyeball 👀 if comfortable)
✅ Impulse Sealer 🔒 / Stapler 📎

Procedure:

1. Preparation: 🧼

Put on your mask 😷 and gloves 🧤.

Clean your workspace thoroughly 🧽.

Use a laminar flow hood 🌀 or SAB to maintain sterility.

Sanitize hands, tools, and the exterior of your colonized spawn bags/jars with 70% isopropyl alcohol.


2. Open Spawn Bag/Jar: ✂️

Carefully open the colonized bag (using scissors ✂️) or jar (untwist the lid) inside the sterile area.

Avoid shaking it too vigorously to prevent contamination 🚫.


3. Open Receiving Bags/Jars:

Carefully open one sterilized grain bag 🛍️ or jar at a time 🕒.


4. Transfer Grains:🌾

Use a sterile cup/tool 🥄 to transfer 1-1 ½ cups of colonized grain into the receiving bag/jar.

Distribute colonized grain evenly for quick and uniform colonization.

If comfortable, eyeball 👀 the measurement.


5. Seal and Shake: 🤝

Reseal the receiving container immediately with an impulse sealer 🔒 or stapler 📎.

Gently shake or roll the jar/bag to distribute the colonized grain evenly 🤲.
6. Repeat: 🔁

Repeat the process for additional jars/bags until complete.


7. Incubate: 🌡️

Place inoculated grain jars/bags in a clean, dark area 🌑 with temperatures between 70-75°F (21-24°C).

Allow 7-14 days for full colonization ⏳.


Tips for Success: 🌟

Minimize airflow in your workspace if using a SAB 🌬️.

Don’t overfill receiving jars; leave space for shaking.

Always sterilize tools between transfers to avoid cross-contamination 🚫🦠.

Discard jars with visible contamination ❌.

🛍Product Links

🫙(12) Pk 16 Oz Wide Mouth Mason Jar

☢️Custom Liquid Culture Lids- https://amzn.to/4kgBwjh

☢️Plastic Wide Mouth Mason Jar Lids- https://amzn.to/4hLOGmO

🔘Filter Paper Sticker/ Rubber Injection Port- https://amzn.to/4ifcvmH

🔳Flowhood-(Ebay)
https://www.ebay.com/itm/373964427364

🌾Grain Bags-(Amazon)
https://amzn.to/41ksZ6y

Whole Oats- (Tractor Supply)
https://www.tractorsupply.com/tsc/pro...

♨️Impulse Sealer-(Amazon)
https://amzn.to/43euN3l

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