How to add Grain Spawn into Master's Mix| Spawning To Bulk (Wood-Loving)

Materials List for Mixing Grain Spawn into Master's Mix in Unicorn Bags

1. Grain Spawn 🌾
Fully colonized grain spawn (e.g., millet, rye berries, or wheat).


2. Master's Mix 🪵🫘
Pre-prepared and sterilized substrate:

50% hardwood sawdust.

50% soybean hulls.


3. Scissors ✂️
To carefully open your grain spawn (if in a bag).


4. Impulse Sealer/Stapler 🔒
To securely seal the bags after mixing.


5. Isopropyl Alcohol (70% or higher) 🧴
For sanitizing gloves, tools, and workspace.


6. Gloves/Mask 🧤😷
Nitrile or latex gloves for sterile handling and a mask to reduce contamination.


7. Clean Workspace 🧼🛡️
Laminar flow hood, still air box (SAB), or a sanitized surface.


8. Storage Area for Colonization 🏠🌡️
Clean, dark environment (70-75°F) for bag incubation.


Step-by-Step: Mixing Grain Spawn into Master's Mix in a Unicorn Bag

1. Sanitize Your Workspace 🧴🧤

Work in a clean environment, preferably near a laminar flow hood or still air box (SAB).

Wear gloves and sanitize them with isopropyl alcohol to reduce contamination risk.


2. Prepare the Unicorn Bag 🛍️

Carefully unfold your Master's Mix bags and open them, so they’re ready to pour the grain spawn in.


3. Add the Grain Spawn 🌾➕🛍️

Break up the colonized grain spawn by massaging the bag or shaking the jar to ensure even distribution.

Carefully open the grain spawn bag or jar and pour the spawn into the Unicorn bag with the substrate.

Target Ratio: Use a 1:5 spawn-to-substrate ratio (e.g., 1 lb grain spawn to 5 lbs Master's Mix).


4. Mix Thoroughly 🌀🤲

Gently mix the grain spawn and substrate by massaging the outside of the bag.

Ensure the grain spawn is evenly distributed throughout the Master's Mix.


5. Seal the Bag 🔒✂️

Once mixed, seal the bag completely using an impulse sealer.


6. Form the Block 🧱

Firmly press your mixture into a nice, compacted block.


7. Incubation/Colonization 🌡️🕰️

Place the sealed bag in a clean, dark, warm environment:

Temperature: 70-75°F.

Time: 7-14 days or until the substrate is fully colonized (mycelium will appear white and even throughout).


Avoid handling or shaking the bag excessively during this phase.


8. Check for Full Colonization ✅🍄‍🟫

Once the substrate is fully white and colonized, it is ready for fruiting conditions.


🛍️ Product Links

Flow Hood 🛡️
https://www.ebay.com/itm/373964427364

3T Unicorn Bags 🛍️
https://amzn.to/4inPU7H

Misting Spray Bottle
https://amzn.to/3DgMvIT

Sterilized Whole Oats 🌾
https://www.needlejuicefungi.com/prod...


Master's Mix 🪵🫘
https://www.needlejuicefungi.com/prod...


Impulse Sealer 🔒
https://amzn.to/4kkG2xp

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