Bacterial Conjugation - Horizontal Gene Transfer Part - I [F+ x F-, Hfr x F-, F' x F-]

Bacterial conjugation is a process of genetic transfer between bacterial cells that requires direct contact between the cell. Conjugation occurs between the same species or different species of cells.

For conjugation, a circular plasmid called F factor is required. F stands for fertility factor. Strains of bacteria containing F factors are called F+ cells and those without it are called F- cells.

F+ cells are the donor cells which produces a structure called pilus to connect with another recipient cell (F-). Pili is a projection forming cytoplasmic bridge that initiates contact with a recipient cell and allows the F DNA to pass through a pore into the recipient cell. One strand of the ds F DNA is transferred and then DNA replication restores the complementary strand in both the donor and recipient cells.

00:38 introduction of horizontal gene transfer,
02:36 introduction of conjugation,
04:13 conjugation between F+ cell x F- cell,
06:41 conjugation between Hfr cell x F- cell,
10:00 conjugation between F' cell x F- cell

F+, F-, Hfr and F’ Cells – What is the Difference? : http://nowiknow.co.in/2017/04/07/f-f-...
Transformation: http://nowiknow.co.in/2017/01/28/bact...
Transduction: http://nowiknow.co.in/2017/01/28/bact...
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