Historic DNA library preparation using the NEBNext® Ultra™ II DNA Library Prep Kit for Illumina
Автор: TAXON-OMICS
Загружено: 2023-02-02
Просмотров: 858
Historic DNA from museum or herbarium specimens is highly fragmented. Generating NGS sequence libraries using customized non-commercial protocols (non-kit) is very time-consuming and thus not suitable for high throughput. We describe a simple kit-based protocol in our video using the NEBNext® Ultra™ II DNA Library Prep Kit.
For more information about the protocol:
https://international.neb.com/product...
▬ Chapters ▬▬▬▬▬▬▬▬▬▬▬▬▬▬▬▬▬▬▬▬▬▬
00:00 Introduction
03:55 Step 1 - NEBNext End Prep
05:48 Step 2 - Adaptor Ligation
08:21 Step 3 - Cleanup of Adaptor-ligated DNA without Size Selection
12:49 Step 4 - PCR Enrichment of Adaptor-ligated DNA
15:45 Step 5 - Cleanup of PCR Reaction
20:36 Step 6 - DNA Sequencing
21:40 Credits
▬ Website ▬▬▬▬▬▬▬▬▬▬▬▬▬▬▬▬▬▬▬▬▬▬
► TAXON-OMICS - "New approaches to discovering and naming biodiversity"
► https://www.taxon-omics.com/
▬ Social Media ▬▬▬▬▬▬▬▬▬▬▬▬▬▬▬▬▬▬▬▬
► Facebook: / dfg.taxonomics
► Twitter: / spp_taxonomics
▬ Music Licences▬▬▬▬▬▬▬▬▬▬▬▬▬▬▬▬▬▬▬
► Fantasy Motion (Loop Ready) by Alexander Nakarada | https://www.serpentsoundstudios.com
Music promoted by https://www.free-stock-music.com
Attribution 4.0 International (CC BY 4.0)
https://creativecommons.org/licenses/...
Доступные форматы для скачивания:
Скачать видео mp4
-
Информация по загрузке:
