Cytek Aurora Panel Design Tutorial

This video gives a detailed process for Cytek Aurora panel design. A shorter video that provides an overview of Aurora panel design tools is available:    • Cytek Aurora Training Part 3: Panel Design...  . Everything in the design tools video is also included in this tutorial video.

Slides can be downloaded here: https://drive.google.com/file/d/1O8BG...

Video content:
0:00 Intro
3:03 Step 1: Info about markers
17:59 Step 2: Pick fluorophores
21:46 What is spillover-spreading error
32:01 Tools for picking fluorophores
35:42 Step 3: Matching markers to fluorophores
54:39 Step 4: Test panel on cells


UChicago Aurora panel design tools: https://voices.uchicago.edu/ucflow/pr...

L Ferrer-Font, C Pellefigues, JU Mayer, S Small, MC Jaimes, KM Price. "Panel Design and Optimization for High-Dimensional Immunophenotyping Assays Using Spectral Flow Cytometry." Current Protocols in Cytometry; https://doi.org/10.1002/cpcy.70 (also: https://www.biorxiv.org/content/10.11...)

Ferrer-Font L, Small SJ, Lewer B, Pilkington KR, Johnston LK, Park LM, Lannigan J, Jaimes MC, Price KM. Panel Optimization for High-Dimensional Immunophenotyping Assays Using Full-Spectrum Flow Cytometry. Curr Protoc. 2021 Sep;1(9):e222. doi: 10.1002/cpz1.222. PMID: 34492732. https://currentprotocols.onlinelibrar...

Cytometry and Antibody Technology Facility
University of Chicago

I have no affiliation with Cytek Biosciences.