HPLC Demystified Part 3 | How to Maintain and Troubleshoot Your HPLC Like a Pro

🎁Download our HPLC Maintenance & Troubleshooting Checklist → https://zfrmz.com/fjn7iuq3bsK0mnFJyqFu

🔧 HPLC Demystified Part 3: How to Maintain and Troubleshoot Your HPLC Like a Pro
Chromatography giving you headaches? Part 3 of the HPLC Demystified series is your lab-side guide to troubleshooting and routine maintenance — explained clearly by Dr. Lee Polite.
Whether you're dealing with air bubbles, leaky seals, noisy baselines, or a worn-out rotor seal, this video walks you through the why, the how, and the fix.

00:00 Intro
00:45 Solvent Bottles - preventing microbial growth
07:55 Degasser - most common way people damage it & first sign of a problem.
10:08 Pump Troubleshooting - Most common problem, good chromatogram
21:40 Pump Maintenance - Replacing pump seals and plugged frit in purge valve
34:41 Autosampler Maintenance - Replacing rotor seals
47:27 Detector Tips

💡 Learn how to:
✅ Detect and fix pump seal issues
✅ Prevent microbial growth in solvent bottles
✅ Avoid damaging your degasser
✅ Replace the rotor seal in your autosampler
✅ Diagnose pressure ripple and baseline noise
✅ Handle light bulb failures in your detector

This is the kind of hands-on wisdom you won’t find in textbooks — straight from someone who runs 12 HPLCs every day.

📌 Watch Part 1 -    • HPLC Demystified Part 1 | Chromatography T...  
📌 Watch Part 2 -    • HPLC Demystified Part 2 | How an HPLC Syst...  

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