HPLC Troubleshooting | Reproducibility Tips
Автор: Axion Labs & Training Institute
Загружено: 2026-01-08
Просмотров: 135
HPLC Reproducibility Problems? It’s NOT Your Method. Here’s What to Check First.
Getting different chromatograms from the same HPLC sample is one of the most frustrating problems in the lab. Different peak areas. Different heights. Sometimes even a different number of peaks.
In this short video, Dr. Lee Polite explains why this is not a method problem and how to quickly diagnose reproducibility issues in your HPLC system without rewriting your method.
You’ll learn:
-Why the same vial should always produce the same chromatogram
-What extra or missing peaks really mean
-How to properly clean an HPLC column to eliminate ghost peaks
-The simple six-injection test that reveals autosampler problems
-How peak area, height, and retention time point to specific hardware issues
If your HPLC data isn’t reproducible, this is the first troubleshooting step you should take.
Visit https://axionlabs.com/ for more troubleshooting tips.
#analyticalchemistry #chromatography #hplcchromatography #axionlabs #chemistryeducation
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